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Generation of Murine Monoclonal Antibodies by Hybridoma Technology

DOI :

10.3791/54832-v

January 2nd, 2017

January 2nd, 2017

39,099 Views

1Department of Biochemistry and Biology, University of Potsdam

An optimized protocol is presented for the generation of monoclonal antibodies based on the hybridoma technology. Mice were immunized with an immunoconjugate. Spleen cells were fused by PEG and an electric impulse with immortal myeloma cells. Antibody-producing hybridoma cells were selected by HAT and antigen-specific ELISA screening.

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Hybridoma Technology

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