JoVE Logo
Faculty Resource Center

Sign In

Temporal Tracking of Cell Cycle Progression Using Flow Cytometry without the Need for Synchronization

DOI :

10.3791/52840-v

August 16th, 2015

August 16th, 2015

18,718 Views

1Centre for Cancer Research, Westmead Millennium Institute for Medical Research and University of Sydney

This protocol describes the use of bromodeoxyuridine (BrdU) uptake to permit the temporal tracking of cells that were in S phase at a specific point in time. Addition of DNA dyes and antibody labeling facilitates detailed analysis of the fate of the S phase cells at later times.

Tags

Cell Cycle Progression

-- Views

Related Videos

article

Flow Cytometry Purification of Mouse Meiotic Cells

article

Measuring Cell Cycle Progression Kinetics with Metabolic Labeling and Flow Cytometry

article

Flow Cytometry-based Purification of S. cerevisiae Zygotes

article

Optimized Staining and Proliferation Modeling Methods for Cell Division Monitoring using Cell Tracking Dyes

article

Cell Tracking Using Photoconvertible Proteins During Zebrafish Development

article

Techniques for the Analysis of Extracellular Vesicles Using Flow Cytometry

article

Synchronization of Caulobacter Crescentus for Investigation of the Bacterial Cell Cycle

article

LED Thermo Flow — Combining Optogenetics with Flow Cytometry

article

Determination of the Relative Cell Surface and Total Expression of Recombinant Ion Channels Using Flow Cytometry

article

Analysis of Cell Suspensions Isolated from Solid Tissues by Spectral Flow Cytometry

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved