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Analysis of Cell Cycle Position in Mammalian Cells

DOI :

10.3791/3491-v

12:19 min

January 21st, 2012

January 21st, 2012

58,656 Views

1Department of Biochemistry, Schulich School of Medicine and Dentistry, University of Western Ontario, 2London Regional Cancer Program, Children's Health Research Institute, and Department of Biochemistry, Schulich School of Medicine and Dentistry, University of Western Ontario

Determining the cell cycle position of a population of cells, or understanding how signals affect proliferation, can be readily measured by flow cytometry using this protocol. We report a simple experimental approach to staining cells and quantifying their position in the cell cycle.

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Cell Cycle Position

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