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Generating CRISPR/Cas9 Mediated Monoallelic Deletions to Study Enhancer Function in Mouse Embryonic Stem Cells

13.9K Views

11:31 min

April 2nd, 2016

DOI :

10.3791/53552-v

April 2nd, 2016


Explore More Videos

CRISPR Cas9

Chapters in this video

0:05

Title

0:56

Transfection

2:40

Culturing Clones

4:23

Allele-specific Primer Design

8:27

Genotyping the Deletion and Analyzing qPCR Results

9:42

Analysis of CRISPR/Cas9 Mediated Deletion in F1 ES Cells

11:05

Conclusion

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