Generation of Centromere-Associated Protein-E CENP-E-/- Knockout Cell Lines using the CRISPR/Cas9 System
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11:49 min
•
June 23rd, 2023
DOI :
June 23rd, 2023
•Meng-Fei Xu1,2, Jie Chen1,2, Yue Xu1,2, Jing-Lian Zhang1,2, Yi Zhou1,2, Jie-Jie He1,2, Shan Wu1,2, Ya-Lan Wei3,4, Zhen-Yu She1,2
1Department of Cell Biology and Genetics, The School of Basic Medical Sciences, Fujian Medical University, 2Key Laboratory of Stem Cell Engineering and Regenerative Medicine, Fujian Province University, 3College of Clinical Medicine for Obstetrics & Gynecology and Pediatrics, Fujian Medical University, 4Medical Research Center, Fujian Maternity and Child Health Hospital
Chapters in this video
0:00
Introduction
2:16
Transfection, Isolation, and Screening of the CENP‐E Knockout HeLa Cells
6:25
Immunofluorescence Staining and High‐Resolution Confocal Microscopy of CRISPR/Cas9‐Mediated CENP‐E Knockout HeLa Cells
9:29
Chromosome Preparation and Karyotype Analysis of CRISPR/Cas9‐Mediated CENP‐E Knockout HeLa Cells
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