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Glutamine Flux Imaging Using Genetically Encoded Sensors

DOI :

10.3791/51657-v

July 31st, 2014

July 31st, 2014

9,436 Views

1Virginia Tech

This article will demonstrate how to monitor glutamine dynamics in live cells using FRET. Genetically encoded sensors allow real-time monitoring of biological molecules at a subcellular resolution. Experimental design, technical details of the experimental settings, and considerations for post-experimental analyses will be discussed for genetically encoded glutamine sensors.

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Keywords Genetically Encoded Sensors

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