Results: Representative Density Gradient- and Bead-isolated CD146+ MSC
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Conclusion
Transcript
The overall goal of this procedure is to invitro characterize CD146 positive resident Mesenchymal Stromal Cell or MSCs isolated from the Rat Lung using a combination of enzymatic digestion, density gradient separation, plastic adherence and bead s
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This protocol describes an isolation technique for obtaining primary lung resident mesenchymal stromal cells from rats, through the use of enzymatic digestion, density gradient separation, plastic adherence and CD146+ magnetic bead selection.