JoVE Logo
Faculty Resource Center

Sign In

Myeloid Cell Isolation from Mouse Skin and Draining Lymph Node Following Intradermal Immunization with Live Attenuated Plasmodium Sporozoites

DOI :

10.3791/53796-v

May 18th, 2016

May 18th, 2016

16,475 Views

1Unité de Biologie et Génétique du Paludisme, Institut Pasteur, 2Unité dImmunobiologie des Cellules Dendritiques, Institut Pasteur

We describe here a protocol for isolating myeloid cells from mouse skin and draining lymph node following intradermal injection of Plasmodium sporozoites. Flow cytometry of collected cells provides a reliable assay to characterize the skin and draining lymph node inflammatory response to the parasite.

Tags

Myeloid Cell Isolation

-- Views

Related Videos

article

Intravital Microscopy of the Inguinal Lymph Node

article

Non-surgical Intratracheal Instillation of Mice with Analysis of Lungs and Lung Draining Lymph Nodes by Flow Cytometry

article

Ex vivo Imaging of T Cells in Murine Lymph Node Slices with Widefield and Confocal Microscopes

article

Murine Superficial Lymph Node Surgery

article

Intravital Imaging of the Mouse Popliteal Lymph Node

article

Isolation of Myeloid Dendritic Cells and Epithelial Cells from Human Thymus

article

Generation of Lymph Node-fat Pad Chimeras for the Study of Lymph Node Stromal Cell Origin

article

Isolation of Murine Lymph Node Stromal Cells

article

Lymphocyte Isolation from Human Skin for Phenotypic Analysis and Ex Vivo Cell Culture

article

Isolation of Infiltrating Leukocytes from Mouse Skin Using Enzymatic Digest and Gradient Separation

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved