JoVE Logo
Faculty Resource Center

Sign In

Circulating MicroRNA Quantification Using DNA-binding Dye Chemistry and Droplet Digital PCR

DOI :

10.3791/54102-v

June 26th, 2016

June 26th, 2016

8,393 Views

1Department of Experimental, Diagnostic and Specialty Medicine - DIMES, University of Bologna, 2Department of Morphology, Surgery and Experimental Medicine, University of Ferrara, 3Department of Life Sciences and Biotechnology, University of Ferrara, 4Department of Morphology, Surgery and Experimental Medicine and Laboratory for Technologies of Advanced Therapies (LTTA), University of Ferrara

A sensitive and accurate method for cell-free microRNAs quantification using a dye-based chemistry and droplet digital PCR technology is described.

-- Views

Related Videos

article

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

article

Fluorescence detection methods for microfluidic droplet platforms

article

Detection and Isolation of Circulating Melanoma Cells using Photoacoustic Flowmetry

article

Diagnosis of Neoplasia in Barrett’s Esophagus using Vital-dye Enhanced Fluorescence Imaging

article

Quantification and Size-profiling of Extracellular Vesicles Using Tunable Resistive Pulse Sensing

article

Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers

article

A Method to Estimate Cadaveric Femur Cortical Strains During Fracture Testing Using Digital Image Correlation

article

Quantifying Microorganisms at Low Concentrations Using Digital Holographic Microscopy (DHM)

article

A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons

article

Bulk Droplet Vitrification for Primary Hepatocyte Preservation

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved