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Laser-assisted Microdissection (LAM) as a Tool for Transcriptional Profiling of Individual Cell Types

DOI :

10.3791/53916-v

May 10th, 2016

May 10th, 2016

9,051 Views

1University of Zürich & Zürich-Basel Plant Science Center

Here we present a protocol for laser-assisted microdissection of specific plant cell types for transcriptional profiling. While the protocol is suitable for different species and cell types, the focus is on highly inaccessible cells of the female germline important for sexual and apomictic reproduction in the crucifer genus Boechera.

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Laser assisted Microdissection

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