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Generation of ESC-derived Mouse Airway Epithelial Cells Using Decellularized Lung Scaffolds

DOI :

10.3791/54019-v

May 5th, 2016

May 5th, 2016

8,441 Views

1Department of Physiology and Experimental Medicine, Peter Gilgan Centre for Research and Learning, Hospital for Sick Children

This protocol efficiently directs mouse embryonic stem cell-derived definitive endoderm to mature airway epithelial cells. This differentiation technique uses 3-dimensional decellularized lung scaffolds to direct lung lineage specification, in a defined, serum-free culture setting.

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