JoVE Logo
Faculty Resource Center

Sign In

Generation of ESC-derived Mouse Airway Epithelial Cells Using Decellularized Lung Scaffolds

DOI :

10.3791/54019-v

May 5th, 2016

May 5th, 2016

8,484 Views

1Department of Physiology and Experimental Medicine, Peter Gilgan Centre for Research and Learning, Hospital for Sick Children

This protocol efficiently directs mouse embryonic stem cell-derived definitive endoderm to mature airway epithelial cells. This differentiation technique uses 3-dimensional decellularized lung scaffolds to direct lung lineage specification, in a defined, serum-free culture setting.

-- Views

Related Videos

article

Electrospun Fibrous Scaffolds of Poly(glycerol-dodecanedioate) for Engineering Neural Tissues From Mouse Embryonic Stem Cells

article

3D Hydrogel Scaffolds for Articular Chondrocyte Culture and Cartilage Generation

article

Epithelial Cell Repopulation and Preparation of Rodent Extracellular Matrix Scaffolds for Renal Tissue Development

article

Fabrication of Decellularized Cartilage-derived Matrix Scaffolds

article

Generation of a Simplified Three-Dimensional Skin-on-a-chip Model in a Micromachined Microfluidic Platform

article

Engineered Lung Tissues Prepared from Decellularized Lung Slices

article

Exposing the Airway and Excising the Vasculature and Alveolar Tissue from a Human and Pig Lung

article

Dissection and Isolation of Region-Specific Decellularized Lung Tissue

article

Human Epithelial Lung Carcinoma Cells Culture and Cell Surface-Enhanced Raman Scattering (SERS) Experiments

article

Decellularized Apple-Derived Scaffolds for Bone Tissue Engineering In Vitro and In Vivo

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved