JoVE Logo
Faculty Resource Center

Sign In

Generation of Airway Epithelial Cell Air-Liquid Interface Cultures from Human Pluripotent Stem Cells

DOI :

10.3791/63882-v

10:46 min

June 14th, 2022

June 14th, 2022

2,739 Views

1Pulmonary Center, Boston University School of Medicine, 2Center for Regenerative Medicine, Boston University and Boston Medical Center, 3Center for Stem Cell and Regenerative Medicine, Brown Foundation Institute of Molecular Medicine, University of Texas Health Science Center

Recent advances in human induced pluripotent stem cell differentiation protocols allow for the stepwise derivation of organ-specific cell types. Here, we provide detailed steps for the maintenance and expansion of iPSC-derived airway basal cells and their differentiation into a mucociliary epithelium in air-liquid interface cultures.

Tags

Airway Epithelial Cell

-- Views

Related Videos

article

Generation of Retinal Organoids from Healthy and Retinal Disease-Specific Human-Induced Pluripotent Stem Cells

article

Targeted Microinjection and Electroporation of Primate Cerebral Organoids for Genetic Modification

article

Optogenetic Inhibition of Rho1-Mediated Actomyosin Contractility Coupled with Measurement of Epithelial Tension in Drosophila Embryos

article

Nuclei Isolation from Mouse Cardiac Progenitor Cells for Epigenome and Gene Expression Profiling at Single-Cell Resolution

article

Author Spotlight: Advancing Lens Biomechanics Research Through a Novel Protocol for Imaging Complex Interdigitations and Protein Staining

article

Author Spotlight: Effect of Left Atrial Ligation on Avian Embryonic Hearts and HLHS Implications

article

Author Spotlight: Understanding DNA Damage Response in Mammalian Oocytes and Preimplantation Embryos

article

Author Spotlight: Investigating Asymmetric Cell Division Dynamics: A Protocol for Live-Imaging of Drosophila Larval Brain Explants

article

Author Spotlight: Cistrome Analysis in Mouse Muscle Stem Cells

article

Author Spotlight: Optimizing iPSC Differentiation for Efficient Production to Generate Kidney Organoids

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved