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Engineered Lung Tissues Prepared from Decellularized Lung Slices

DOI :

10.3791/63151-v

8:01 min

January 21st, 2022

January 21st, 2022

2,552 Views

1Department of Biomedical Engineering, Yale University, 2Yale School of Medicine, 3Department of Cellular and Molecular Physiology, Yale School of Medicine, 4Department of Anesthesiology, Yale School of Medicine

This protocol describes a method to generate reproducible, small-scale engineered lung tissues, by repopulating decellularized precision-cut lung slices with alveolar epithelial type 2 cells, fibroblasts, and endothelial cells.

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Engineered Lung Tissues

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