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One-step Negative Chromatographic Purification of Helicobacter pylori Neutrophil-activating Protein Overexpressed in Escherichia coli in Batch Mode

DOI :

10.3791/54043-v

June 18th, 2016

June 18th, 2016

9,513 Views

1Institute of Molecular and Cellular Biology, National Tsing Hua University, 2Department of Life Science, National Tsing Hua University

A high yield method for one-step negative purification of recombinant Helicobacter pylori neutrophil-activating protein (HP-NAP) overexpressed in Escherichia coli by using diethylaminoethyl resins in batch mode is described. HP-NAP purified by this method is beneficial for the development of vaccines, drugs, or diagnostics for H. pylori-associated diseases.

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