JoVE Logo
Faculty Resource Center

Sign In

Assessment of Myofilament Ca2+ Sensitivity Underlying Cardiac Excitation-contraction Coupling

DOI :

10.3791/54057-v

August 1st, 2016

August 1st, 2016

9,224 Views

1Department of Physiology & Biomedical Sciences, Ischemic/hypoxic Disease Institute, Seoul National University College of Medicine, 2Yan Bian University Hospital, 3Institute of Cardiovascular Sciences, University of Manchester

This paper describes a protocol that assesses the changes of myofilament Ca2+ sensitivity during contraction in isolated cardiac myocytes from rat heart. Together with cardiac electrophysiology, systolic/diastolic cytosol Ca2+ levels and contraction/relaxation, this measurement is imperative in underpinning the mechanisms mediating cardiac excitation-contraction coupling in healthy and diseased hearts.

-- Views

Related Videos

article

Optical Mapping of Langendorff-perfused Rat Hearts

article

Semi-automated Optical Heartbeat Analysis of Small Hearts

article

Efficient Derivation of Human Cardiac Precursors and Cardiomyocytes from Pluripotent Human Embryonic Stem Cells with Small Molecule Induction

article

Ex Vivo Assessment of Contractility, Fatigability and Alternans in Isolated Skeletal Muscles

article

Biochemical and High Throughput Microscopic Assessment of Fat Mass in Caenorhabditis Elegans

article

Measuring Intracellular Ca2+ Changes in Human Sperm using Four Techniques: Conventional Fluorometry, Stopped Flow Fluorometry, Flow Cytometry and Single Cell Imaging

article

Live Imaging Assay for Assessing the Roles of Ca2+ and Sphingomyelinase in the Repair of Pore-forming Toxin Wounds

article

Manual Isolation of Adipose-derived Stem Cells from Human Lipoaspirates

article

A Sensitive and Specific Quantitation Method for Determination of Serum Cardiac Myosin Binding Protein-C by Electrochemiluminescence Immunoassay

article

Assessment of Calcium Sparks in Intact Skeletal Muscle Fibers

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved