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In Vitro Assessment of Cardiac Function Using Skinned Cardiomyocytes

DOI :

10.3791/60427-v

8:19 min

June 22nd, 2020

June 22nd, 2020

5,356 Views

1Unidade de Investigação Cardiovascular, Departamento de Cirurgia e Fisiologia, Faculdade de Medicina, Universidade do Porto, 2Department of Physiology, Kilimanjaro Christian Medical University College

This protocol aims to describe step-by-step the technique of extraction and assessment of cardiac function using skinned cardiomyocytes. This methodology allows measurement and acutemodulation of myofilament function using small frozen biopsies that can be collected from different cardiac locations, from mice to men.

Tags

In Vitro Assessment

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