Cryo-fixation of Leaf Tissues by High-pressure Freezing
3:43
Freeze Fracture and Double-layer Coating
7:10
Cryo-scanning Electron Microscopy
10:52
Results: Cryo-scanning Electron Microscopy Analysis of Photosynthetic Membranes in Freeze-fractured Leaf Tissues
12:57
Conclusion
Transcript
The overall goal of this technique is to study the supramolecular organization of photosynthetic membranes using cryo-scanning electron microscopy of freeze-fractured leaf tissue samples. This method can help answer key questions in cell biology,
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Here we describe a procedure for studying freeze-fractured plant tissues. High-pressure frozen leaf samples are freeze-fractured and double-layer coated, yielding well preserved frozen-hydrated samples that are imaged using the cryo-scanning electron microscope at high magnifications with minimal beam damage.