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Highly Sensitive and Rapid Fluorescence Detection with a Portable FRET Analyzer

DOI :

10.3791/54144-v

October 1st, 2016

October 1st, 2016

8,742 Views

1Synthetic Biology & Bioengineering Research Center, Korea Research Institute of Bioscience and Biotechnology, 2College of Interdisciplinary & Creative Studies, Konyang University, 3Biosystems and Bioengineering Program, University of Science and Technology

This protocol describes the rapid and highly sensitive quantification of Förster resonance energy transfer (FRET) sensor data using a custom-made portable FRET analyzer. The device was used to detect maltose within a critical temperature range that maximizes detection sensitivity, enabling practical and efficient assessment of sugar content.

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