JoVE Logo
Faculty Resource Center

Sign In

Mucin Agarose Gel Electrophoresis: Western Blotting for High-molecular-weight Glycoproteins

DOI :

10.3791/54153-v

June 14th, 2016

June 14th, 2016

24,088 Views

1Marsico Lung Institute/CF Center, University of North Carolina at Chapel Hill, 2Telethon Kids Institute, University of Western Australia, 3Department of Pediatrics, University of North Carolina at Chapel Hill

Mucins are high-molecular-weight glycoconjugates, with size ranging from 0.2 to 200 megadalton (MDa). As a result of their size, mucins do not penetrate conventional polyacrylamide gels and require larger pores for separation. We provide a detailed protocol for mucin agarose gel electrophoresis to assess relative quantification and study polymer assembly.

Tags

Mucin Agarose Gel Electrophoresis

-- Views

Related Videos

article

Denaturing Gradient Gel Electrophoresis (DGGE)

article

Western Blotting Using the Invitrogen NuPage Novex Bis Tris MiniGels

article

Assessing Neural Stem Cell Motility Using an Agarose Gel-based Microfluidic Device

article

Screening for Amyloid Aggregation by Semi-Denaturing Detergent-Agarose Gel Electrophoresis

article

Denaturing Urea Polyacrylamide Gel Electrophoresis (Urea PAGE)

article

Extraction of High Molecular Weight Genomic DNA from Soils and Sediments

article

Blue Native Polyacrylamide Gel Electrophoresis (BN-PAGE) for Analysis of Multiprotein Complexes from Cellular Lysates

article

Visualization of UV-induced Replication Intermediates in E. coli using Two-dimensional Agarose-gel Analysis

article

Western Blotting: Sample Preparation to Detection

article

Extraction of High Molecular Weight DNA from Microbial Mats

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved