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Mucin Agarose Gel Electrophoresis: Western Blotting for High-molecular-weight Glycoproteins

25.3K Views

09:24 min

June 14th, 2016

DOI :

10.3791/54153-v

June 14th, 2016


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Mucin

Chapters in this video

0:05

Title

0:43

TAE-SDS Agarose Gel Preparation

2:02

Sample Loading and Electrophoresis Separation

3:32

Agarose Gel Reduction

4:08

Vacuum Blotter Assembly and Sample Transfer to a Nitrocellulose Membrane

6:33

Membrane Blocking and Detection

8:08

Results: Enhanced Analysis of High-molecular-weight Glycoproteins

8:45

Conclusion

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