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JoVE Journal

Immunology and Infection

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Rapid, Seamless Generation of Recombinant Poxviruses using Host Range and Visual Selection

5.2K Views

09:25 min

May 24th, 2020

May 24th, 2020

5,154 Views

0:04

Introduction

1:03

Recombination Vector Generation

3:47

Recombinant Virus Generation

6:31

Results: Representative Recombinant Poxvirus Generation

8:36

Conclusion

Transcript

Our method uses host range selection and fluorescent markers for the efficient and seamless generation of recombinant vaccina virus in a variety of cell types. This technique combines the speed of homologous recombination with the scarless nature

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This is a method to generate “scarless” recombinant vaccinia viruses using host-range selection and visual identification of recombinant viruses.

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