Chinese Hamster Ovary (CHO)-Intercellular Adhesion Molecule (ICAM) Cell Plating
1:50
T Cell Preparation
2:46
Input/Output Hosing and Syringe Pump Preparation
3:59
Flow Chamber Loading and Cell Stimulation
5:53
Results: SDF-1alpha Induced T Cell Adhesion to ICAM-1 Under Shear Stress
6:51
Conclusion
Transkript
The overall goal of this assay is to determine the effects of treatments of interest on cellular adhesion under shear stress conditions. This method can help answer key questions in the immune cell signaling field, such as, what factors mediate in
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This flow adhesion assay provides a simple, high impact model of T cell-epithelial cell interactions. A syringe pump is used to generate shear stress, and confocal microscopy captures images for quantification. The goal of these studies is to effectively quantify T cell adhesion using flow conditions.