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Applying Fluorescence Resonance Energy Transfer (FRET) to Examine Effector Translocation Efficiency by Coxiella burnetii during siRNA Silencing

10.6K Views

10:29 min

July 6th, 2016

DOI :

10.3791/54210-v

July 6th, 2016


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FRET

Chapters in this video

0:05

Title

1:02

Reverse Transfection of siRNA and Seeding of HeLa 229 Cells

4:25

Infection of Reverse Transfected Cells

5:39

Addition of BlaM Substrate to Determine Level of Translocation

8:23

Results: FRET Measurements of Coxiella burnetti Effector Translocation Efficiency in siRNA Silenced HeLa Cells

9:37

Conclusion

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