Methods Article

Testing Cancer Immunotherapeutics in a Humanized Mouse Model Bearing Human Tumors

2.9K Views

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15:24 min

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December 16th, 2022

DOI :

10.3791/64606-v

December 16th, 2022

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Jordi M. Lanis*¹, Matthew S. Lewis*¹, Hannah Strassburger¹, Kristina Larsen¹, Stacey M. Bagby², Adrian T. A. Dominguez², Juan A. Marín-Jiménez³, Roberta Pelanda¹, Todd M. Pitts², Julie Lang¹

¹Department of Immunology and Microbiology, School of Medicine, University of Colorado Denver Anschutz Medical Campus, ²Division of Oncology, School of Medicine, University of Colorado Denver Anschutz Medical Campus, ³Department of Medical Oncology, Catalan Institute of Oncology (ICO-L'Hospitalet)

Summary

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Cancer Immunotherapy

Humanized Mouse Model

Immunological Analysis

Magnetic Cell Separator

Chapters in this video

0:04

Introduction

1:06

Generation of Human Immune System Mice

4:59

Testing Human Chimerism in Blood

8:36

Harvesting of Mouse Tissues and Tumors

11:18

Results: Evaluation of Chimerism, Tumor Growth Measurements, and Analysis of HIS Cells

14:17

Conclusion

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