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High-resolution Time-lapse Imaging and Automated Analysis of Microtubule Dynamics in Living Human Umbilical Vein Endothelial Cells

DOI :

10.3791/54265-v

10:25 min

August 13th, 2016

August 13th, 2016

10,611 Views

1Department of Biological Sciences, University of the Sciences in Philadelphia

Protocols for Human Umbilical Vein Endothelial Cell (HUVEC) culture, transient transfection of fluorescently-labeled markers of microtubule growth, live-cell imaging and automated analysis of interphase microtubule growth dynamics are detailed.

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High resolution

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