JoVE Logo

Sign In

Microtubule Plus-End Dynamics Visualization in Huntington's Disease Model based on Human Primary Skin Fibroblasts

2.8K Views

10:38 min

January 8th, 2022

DOI :

10.3791/62963-v

January 8th, 2022


Explore More Videos

Huntington s Disease

Chapters in this video

0:05

Introduction

0:44

Protocol

1:02

Obtaining a Primary Culture of Human Skin Fibroblasts

2:14

Cell Cultivation

4:45

Transfection

6:46

Preparing for Imaging

8:08

Setting the Imaging Parameters

9:45

Results

10:13

Conclusion

Related Videos

article

15:09

The Use of Primary Human Fibroblasts for Monitoring Mitochondrial Phenotypes in the Field of Parkinson's Disease

16.7K Views

article

07:43

Modeling Charcot-Marie-Tooth Disease In Vitro by Transfecting Mouse Primary Motoneurons

6.9K Views

article

08:44

Quantitative Cell Biology of Neurodegeneration in Drosophila Through Unbiased Analysis of Fluorescently Tagged Proteins Using ImageJ

9.3K Views

article

08:27

Single Synapse Indicators of Glutamate Release and Uptake in Acute Brain Slices from Normal and Huntington Mice

6.1K Views

article

07:43

In vivo Assessment of Microtubule Dynamics and Orientation in Caenorhabditis elegans Neurons

3.0K Views

article

08:59

Real-Time Fluorescent Measurement of Synaptic Functions in Models of Amyotrophic Lateral Sclerosis

2.5K Views

article

08:07

Label-Free Non-Linear Optics for the Study of Tubulin-Dependent Defects in Central Myelin

1.8K Views

article

10:25

High-resolution Time-lapse Imaging and Automated Analysis of Microtubule Dynamics in Living Human Umbilical Vein Endothelial Cells

11.0K Views

article

08:04

Using Drosophila Larval Neuromuscular Junction and Muscle Cells to Visualize Microtubule Network

2.1K Views

article

08:09

CUBIC Protocol Visualizes Protein Expression at Single Cell Resolution in Whole Mount Skin Preparations

11.5K Views

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2025 MyJoVE Corporation. All rights reserved