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Patch Clamp Recordings on Intact Dorsal Root Ganglia from Adult Rats

DOI :

10.3791/54287-v

September 29th, 2016

September 29th, 2016

13,362 Views

1Department of Oral and Maxillofacial Surgery, University of California, San Francisco, 2Department of Anatomy, University of California, San Francisco

This manuscript describes how to prepare intact dorsal root ganglia for patch clamp recordings. This preparation maintains the microenvironment for neurons and satellite glial cells, thus avoiding the phenotypic and functional changes seen using dissociated DRG neurons.

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