Results: Representative Flow Cytometric and Gene Expression Analysis of Fos-expressing Neurons from Fresh and Frozen Brain Tissue
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Transcript
The overall goal of this procedure is to dissociate Fos-expressing neurons from fresh or frozen adult rat brain tissue for subsequent fluorescence activated cell sorting, or FACS. This method can be used to isolate neuronal cells of interest for i
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Here we present a Fluorescence Activated Cell Sorting (FACS) protocol to study molecular alterations in Fos-expressing neuronal ensembles from both fresh and frozen brain tissue. The use of frozen tissue allows FACS isolation of many brain areas over multiple sessions to maximize the use of valuable animal subjects.