September 20th, 2016
•We present a protocol for identifying and quantifying the components in mixtures of species possessing similar proteins. Mass spectrometry detects peptides for identification, and gives relative quantitation by ratios of peak areas. As a tool food for fraud detection, the method can detect 1% horse in beef.
Tags
Related Videos
Time-resolved ElectroSpray Ionization Hydrogen-deuterium Exchange Mass Spectrometry for Studying Protein Structure and Dynamics
Resolving Affinity Purified Protein Complexes by Blue Native PAGE and Protein Correlation Profiling
Sample Preparation for Mass Spectrometry-based Identification of RNA-binding Regions
The Determination of Protease Specificity in Mouse Tissue Extracts by MALDI-TOF Mass Spectrometry: Manipulating PH to Cause Specificity Changes
Proofreading and DNA Repair Assay Using Single Nucleotide Extension and MALDI-TOF Mass Spectrometry Analysis
Nitropeptide Profiling and Identification Illustrated by Angiotensin II
Sample Preparation for Probe Electrospray Ionization Mass Spectrometry
Quantitative Analysis of the Cellular Lipidome of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry
Rapid Determination of Antibody-Antigen Affinity by Mass Photometry
Semi-Quantitative Analysis of Peptidoglycan by Liquid Chromatography Mass Spectrometry and Bioinformatics
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved