This protocol described a method for red tumor cell enumeration, and the clinical CTC isolation. To begin, culture the tumor cells MCF-7, MDA-MB-231, and HeLa in a cell-culture flask at one times 10 to the fifth in one-millimeter of DMEM, suppleme
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The clinical microfluidic chip is an important biomedical analysis technique that simplifies clinical patient blood sample preprocessing and immunofluorescently stains circulating tumor cells (CTCs) in situ on the chip, allowing the rapid detection and identification of a single CTC.