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Procedure for Adaptive Laboratory Evolution of Microorganisms Using a Chemostat

DOI :

10.3791/54446-v

September 20th, 2016

September 20th, 2016

13,595 Views

1Super-Bacteria Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 2Microbiomics and Immunity Research Center, Korea Research Institute of Bioscience and Bioengineering (KRIBB), 3Department of Biotechnology, The Catholic University of Korea

Here, we present a protocol to obtain adaptive laboratory evolution of microorganisms under conditions using chemostat culture. Also, genomic analysis of the evolved strain is discussed.

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