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Producing Gene Deletions in Escherichia coli by P1 Transduction with Excisable Antibiotic Resistance Cassettes

DOI :

10.3791/58267-v

September 1st, 2018

September 1st, 2018

15,764 Views

1Evolution and Genetics, Department of Biosciences, University of Oslo

Here we present a protocol for the use of pre-existing antibiotic resistance-cassette deletion constructs as a basis for making deletion mutants in other E. coli strains. Such deletion mutations can be mobilized and inserted into the corresponding locus of a recipient strain using P1 bacteriophage transduction.

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