9.5K Views
•
11:37 min
•
February 26th, 2019
DOI :
February 26th, 2019
•Chapters in this video
0:04
Title
1:45
Positive and Negative Screening for Evolving Cas9
4:47
Delivery of Cas9 as an RNP with Truncated sgRNA
6:17
RNP Delivery
7:27
Transfection of Plasmids Encoding Sniper-Cas9 and sgRNA
9:01
Targeted Deep Sequencing for the Analysis of On-target and Potential Off-target Sites
9:29
Results: Analysis of Sniper-screen, On-target and Off-target Activities
10:37
Conclusion
Related Videos
Procedure for Adaptive Laboratory Evolution of Microorganisms Using a Chemostat
14.1K Views
CRISPR/Cas9-mediated Targeted Integration In Vivo Using a Homology-mediated End Joining-based Strategy
14.7K Views
CRISPR/Cas9 Gene Editing to Make Conditional Mutants of Human Malaria Parasite P. falciparum
9.8K Views
Endogenous Protein Tagging in Human Induced Pluripotent Stem Cells Using CRISPR/Cas9
25.9K Views
CRISPR/Cas9 Ribonucleoprotein-mediated Precise Gene Editing by Tube Electroporation
13.7K Views
Introducing a Gene Knockout Directly Into the Amastigote Stage of Trypanosoma cruzi Using the CRISPR/Cas9 System
8.9K Views
Generation of Defined Genomic Modifications Using CRISPR-CAS9 in Human Pluripotent Stem Cells
8.1K Views
Investigation of the Transcriptional Role of a RUNX1 Intronic Silencer by CRISPR/Cas9 Ribonucleoprotein in Acute Myeloid Leukemia Cells
7.3K Views
Cell Surface Receptor Identification Using Genome-Scale CRISPR/Cas9 Genetic Screens
14.2K Views
In Vitro Directed Evolution of a Restriction Endonuclease with More Stringent Specificity
7.1K Views
Copyright © 2025 MyJoVE Corporation. All rights reserved