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Generation of Two-color Antigen Microarrays for the Simultaneous Detection of IgG and IgM Autoantibodies

DOI :

10.3791/54543-v

September 15th, 2016

September 15th, 2016

12,612 Views

1Multi-Organ Transplant Program, University Health Network, 2Institute of Medical Science, University of Toronto, 3Divison of Neurosurgery, University Health Network, 4Division of Cardiac Surgery, University Health Network

We describe here a method to generate customizable antigen microarrays that can be used for the simultaneous detection of serum IgG and IgM autoantibodies from humans and mice. These arrays allow for high-throughput and quantitative detection of antibodies against any antigens or epitopes of interest.

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