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Detection and Enrichment of Rare Antigen-specific B Cells for Analysis of Phenotype and Function

DOI :

10.3791/55382-v

February 16th, 2017

February 16th, 2017

11,112 Views

1Department of Immunology and Microbiology, University of Colorado School of Medicine, 2Department of Microbiology, Immunology, and Pathology, Colorado State University, 3Barbara Davis Center for Childhood Diabetes, University of Colorado School of Medicine, 4Department of Biomedical Research, National Jewish Health

A simple yet effective method that employs magnetic nanoparticles to detect and enrich antigen-reactive B cells for functional and phenotypic analysis is described.

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Detection

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