February 16th, 2017
•A simple yet effective method that employs magnetic nanoparticles to detect and enrich antigen-reactive B cells for functional and phenotypic analysis is described.
Tags
Related Videos
Murine Model of CD40-activation of B cells
Antigen Specific In Vivo Killing Assay using CFSE Labeled Target Cells
Measurement of Antibody Effects on Cellular Function of Isolated Cardiomyocytes
Peptide:MHC Tetramer-based Enrichment of Epitope-specific T cells
Killer Artificial Antigen Presenting Cells (KaAPC) for Efficient In Vitro Depletion of Human Antigen-specific T Cells
Detection of True IgE-expressing Mouse B Lineage Cells
Development of Stem Cell-derived Antigen-specific Regulatory T Cells Against Autoimmunity
In Situ MHC-tetramer Staining and Quantitative Analysis to Determine the Location, Abundance, and Phenotype of Antigen-specific CD8 T Cells in Tissues
In Vivo Assay for Detection of Antigen-specific T-cell Cytolytic Function Using a Vaccination Model
Generation of Discriminative Human Monoclonal Antibodies from Rare Antigen-specific B Cells Circulating in Blood
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved