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Reliably Engineering and Controlling Stable Optogenetic Gene Circuits in Mammalian Cells

DOI :

10.3791/62109-v

July 6th, 2021

July 6th, 2021

1,962 Views

1Biomedical Engineering Department, Stony Brook University, 2Laufer Center for Physical and Quantitative Biology, Stony Brook University, 3Stony Brook Medical Scientist Training Program

Reliably controlling light-responsive mammalian cells requires the standardization of optogenetic methods. Toward this goal, this study outlines a pipeline of gene circuit construction, cell engineering, optogenetic equipment operation, and verification assays to standardize the study of light-induced gene expression using a negative-feedback optogenetic gene circuit as a case study.

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