Fluorescence Microscopy of Light-Induced Engineered Cells
4:32
Flow Cytometry of Light-Induced Engineered Cells
5:14
Immunofluorescence of Gene Circuit Components
7:16
Results: Gene Expression Analysis in Engineered Optogenetic Cell Lines
8:49
Conclusion
Transcript
The standardized optogenetic protocol is designed for labs interested in using light as the stimulus to control cellular properties in engineered mammalian cell systems. This protocol is easy to implement in labs otherwise unfamiliar with optogene
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Reliably controlling light-responsive mammalian cells requires the standardization of optogenetic methods. Toward this goal, this study outlines a pipeline of gene circuit construction, cell engineering, optogenetic equipment operation, and verification assays to standardize the study of light-induced gene expression using a negative-feedback optogenetic gene circuit as a case study.