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Engineering and Characterization of an Optogenetic Model of the Human Neuromuscular Junction

DOI :

10.3791/63759-v

April 14th, 2022

April 14th, 2022

2,122 Views

1Department of Biomedical Engineering, Columbia University, 2Department of Medicine, Columbia University, 3Gladstone Institutes, 4College of Dental Medicine, Columbia University

We describe a reproducible, automated, and unbiased imaging system for characterizing neuromuscular junction function using human engineered skeletal muscle tissue and optogenetic motoneurons. This system allows for the functional quantification of neuromuscular connectivity over time and detects diminished neuromuscular function caused by neurotoxins and myasthenia gravis patient serum.

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Keywords Optogenetic

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