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Nuclear Magnetic Resonance Spectroscopy for the Identification of Multiple Phosphorylations of Intrinsically Disordered Proteins

DOI :

10.3791/55001-v

December 27th, 2016

December 27th, 2016

18,340 Views

1UMR8576, CNRS, Lille University, 2UMR-S1172, INSERM CNRS, Lille University

We describe here a method to identify multiple phosphorylations of an intrinsically disordered protein by Nuclear Magnetic Resonance Spectroscopy (NMR), using Tau protein as a case study. Recombinant Tau is isotopically enriched and modified in vitro by a kinase prior to data acquisition and analysis.

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Keywords Nuclear Magnetic Resonance Spectroscopy

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