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Establishment of Proliferative Tetraploid Cells from Nontransformed Human Fibroblasts

DOI :

10.3791/55028-v

January 8th, 2017

January 8th, 2017

7,128 Views

1Division of Morphological Science, Biomedical Research Center, Saitama Medical University, 2Department of Pathology, International Medical Center, Saitama Medical University

Although proliferative polyploid cells are necessary to analyze chromosomal instability of polyploid cells, creating such cells from nontransformed human cells is not easy. The present report describes relatively simple procedures to establish proliferative tetraploid cells free of a diploid population from normal human fibroblasts.

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Proliferative Tetraploid Cells

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