We thank M. Forney, R. Maged, and K. Hellwinkle for data collection and two anonymous reviewers for comments on a previous version of this manuscript. This research was supported by an NSF award to LDL (IOS-0918268).

All procedures were approved by the Penn State University&#160;Institutional Animal Care and Use Committee (IACUC - Protocol ID: 43242) and adhered to all local, state, and federal regulations.
1. Preparation
<ol>
	<li>Purchase or construct the Barnes maze, assuring that goal holes are appropriately sized for the species of interest. For this protocol, use seven adult side-blotched lizards (Uta stansburiana). Determine adequate sample size for studies using other species.
	<ol>
		<li>If constructing the Barnes maze, purchase any standard, nonporous round table (106 cm in diameter), elevated 76.2 cm off the floor.</li>
		<li>Using a tape measure, mark 10 equidistant points spaced 26 cm apart around the perimeter of the table and 6.35 cm from the edge of the table. Use 10 holes whereas rodent mazes typically have more holes. Doing so allows for a reduction in the number of escape options.</li>
		<li>Using a hole saw bit (2.54 cm in diameter), drill out all 10 goal holes and sand any sharp edges.</li>
		<li>Mount four shelf support pegs under each hole with screws, using a home enclosure as a guide for dimensions.</li>
	</ol>
	</li>
	<li>Place the maze in a quiet room with bright overhead lighting and spatial cues available for navigation (doors, cabinets, etc.).</li>
	<li>Mount an auto focus, wide-angle lens webcam (11.3 cm x 3.99 cm) or other suitable camera directly above the maze. Be certain that the field of view from the video feed encompasses the entire maze.</li>
	<li>Place the computer and observer chair at least 1 m from the maze.</li>
	<li>Mark the legs of the maze on the floor with a permanent marker or tape. This allows for correction of any minute movement of the maze over the course of the trials.</li>
	<li>Randomly assign one of the holes as &#34;1&#34; and number the other holes consecutively through &#34;10&#34;. With a permanent marker, write numbers along the side of the outside of the maze for ease of reference. Ensure that the numbers are not visible to an animal within the maze.</li>
	<li>Randomly assign goal holes to the subjects using a random number generator.</li>
</ol>
2. Training Trials
NOTE: Reptiles have a preferred body temperature that can be obtained from the literature. If animals become sluggish over multiple training trials, the maze may be too cool and this may affect behavior. A small space heater or heat tape on the underside of the maze can adequately increase maze surface temperature, the best thermal indication of body temperature, to maintain optimum body temperature and behavioral performance.
<ol>
	<li>If subjects are housed outside of the testing room, bring animals in their home enclosures into the room at least 30 min before testing. Be certain to provide heat in home enclosures (e.g., heat lamps, heat tape) in order to maintain body temperature before testing.
	<ol>
		<li>Open tracking software and webcam application for the video feed. The custom tracking software was written for Matlab and the Image Processing toolbox; other tracking software is commercially available.</li>
		<li>Evaluate the field of view from the video feed. If the maze is not in the field of view, check that the maze is within the marks on the floor.</li>
		<li>Open the worksheet containing information as to the date, start/end time of training trials, subject ID, assigned goal hole, number of training trials run, notes, and observer&#39;s initials.</li>
		<li>Randomize the order in which subjects will be tested. Test subjects in a systematic manner.</li>
	</ol>
	</li>
	<li>Remove the first subject from its home cage and gently place the subject in the middle of the maze. Place a plastic tub over the animal; doing so prevents unconscious orientation bias by the researcher. Allow 30 s to acclimate.</li>
	<li>Mount the animal&#39;s home cage under the maze, directly below the assigned goal hole, by sliding the top of the enclosure into the peg mounts. Be certain that the basking rock in the home cage is directly under the hole, which will serve as a perch when the animal descends into the hole. At this point, the use of thermal or chemical cues of the home enclosure for use in navigation is irrelevant.
	<ol>
		<li>Rodent mazes&#160;use a clean enclosure as an escape for the subject. However, encourage entry into the hole during training trials by mounting the animal&#39;s home enclosure under the maze.</li>
	</ol>
	</li>
	<li>Gently remove the plastic tub from the top of the maze.</li>
	<li>Start recording of the training trial with the webcam application.
	<ol>
		<li>Allow the subject to explore the maze for 10 min. If anything atypical occurs, note this in the worksheet.</li>
		<li>If the subject falls or runs off the table, gently return the subject to the middle of the maze. Continue timing and recording if the animal is out of the maze for 30 s or less. If the subject is out of the maze for more than 30 s, abort the trial. Record this information in the notes section of the workbook.</li>
		<li>If the subject descends into the goal hole unassisted, consider the trial as over at that time.</li>
		<li>If the subject does not descend into the goal hole, consider the trial as over after 10 min have elapsed.</li>
	</ol>
	</li>
	<li>If the subject does not descend into the goal hole after 10 min, by hand gently guide the animal, head first, into the appropriate goal hole.</li>
	<li>Stop video and tracking software. Save video footage.</li>
	<li>Allow the subject to rest for at least 2 min in its home enclosure while the enclosure is still mounted under the maze.</li>
	<li>Using a spray bottle and paper towels, clean the top of the maze with a diluted soap mixture of 1:10 soap to water. Doing so precludes the use of chemical cues when navigating the maze. Some species, particularly those that make heavy use of chemosensory information, necessitate additional rinsing of the maze with hot water to eliminate all scent cues.</li>
	<li>Remove the enclosure from under the maze and either repeat the training trial with the same individual or a new individual. Each subject can be run in up to 5 training trials per day, with 30 min&#160;interval between an individual&#39;s trials.</li>
	<li>Repeat this procedure until a subject descends into its goal hole unassisted, in 3 different training trials, indicating that learning of the goal location has occurred. The three training trials do not need to be consecutive. If a subject reaches this criterion, the subject moves to the probe trial in order to assess spatial memory.</li>
</ol>
3. Probe Trials
NOTE: Once a subject reaches criterion, the subject has learned how to navigate to the goal. However, at this point, it is still unclear if the subject is navigating using a spatial strategy or some other navigational strategy. Probe trials test for this and should be performed the day after the subject reaches criterion in the training trials.
<ol>
	<li>Rotate the maze 180&#176;, assuring that the legs of the maze are within the marks on the floor. Rotation of the maze allows for local cues that directly identify the goal to be in direct conflict with the more stable spatial cues.</li>
	<li>Repeat steps 2.1 - 2.5.2, omitting step 2.3 of mounting the subject&#39;s home enclosure under the maze. Doing so precludes the use of olfactory or other cues emanating from the home enclosure during maze exploration.</li>
	<li>During probe trials, subjects are free to explore the maze for the full 10 min to assess exploratory behavior. After 10 min, stop video and tracking software.</li>
	<li>Remove the subject from the maze and return it to its home cage.</li>
	<li>Clean the top of the maze with the diluted soap mixture.</li>
</ol>
4. Behavioral Measures
NOTE: For training trials, include behavioral measures such as latency to arrive at the goal hole, number of errors made (investigation of a nongoal hole; the animal&#39;s snout must be within 1 cm of the hole), and proportion of time spent in the correct quadrant of the maze.
<ol>
	<li>For training trials, determine a search strategy. A direct strategy is defined as descending into the goal hole with fewer than 3 errors. A serial strategy is examination of 3 or more consecutive maze holes along the perimeter of the maze. A random strategy is non-serial examination of maze holes with 3 or more errors. 
	NOTE: For probe trials, behavioral measures include latency to arrive at the spatially correct hole, number of errors made before investigating the spatially correct hole, and the proportion of time spent in the spatially correct quadrant of the maze.</li>
	<li>For a more stringent assessment of non-random choice of the spatially correct goal hole, employ sampling without replacement when calculating chance rate.</li>
</ol>

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The authors declare that they have no competing financial interests.

When experimentally testing for spatial learning and memory, there are several important conceptual issues that are addressed in some of the major steps in the protocol. First, subjects must demonstrate that they are learning the location of the goal hole over the course of the training trials. Attaining the preset criterion demonstrates that learning of the goal hole location has occurred. If subjects do not learn the location of the goal hole, there is no feasible way to then determine a navigational strategy. If anima...

Many neurodegenerative diseases such as Alzheimer&#39;s present with a progressive decline in cognitive ability, typically concurrent with degradation of the brain1-4. To test for the influence of brain injury and degradation on cognitive processes, clinical research has leveraged the advantages of model rodent species and standardization of testing apparatus and protocol. In particular, spatial learning and memory processes have been assessed via several standard paradigms such as the Morris water maze, Barnes maze, and radial arm maze (for a comprehensive review of these and other paradigms, see 5,6). The rich history of these spatial learning and memory paradigms has proven quite successful, allowing researchers to understand many of the facets and nuances of the relationship among human memory, brain function, and disease.
While assessment of cognitive processes has been examined in clinical research for quite some time, research directed towards the cognitive abilities of nonmodel species is relatively new. Researchers studying cognition in nonmodel species are typically interested in the ecological and evolutionary relevance of cognitive processes, particularly in the context of survival and reproduction. Some studies in reptiles have suggested that advanced cognitive abilities, in particular spatial memory, may underlie some behaviors, particularly those concerning navigation and orientation. However, while many studies have demonstrated that reptiles can reorient after displacement7,8, the cognitive mechanisms underlying reorientation behavior have not yet been teased apart. Because of this, some studies have attempted to experimentally assess the importance of spatial learning and memory during navigation9-17. The methodology in these studies are predominantly modeled after rodent paradigms and protocols, sometimes modified for use in reptiles, but these studies have had variable success in assessing spatial memory. Some studies have demonstrated spatial learning and memory in some species11-17 while other studies found no evidence of such9,10. Thus, the role or existence of spatial learning and memory during navigation in reptiles is still unclear.
One issue that may be problematic when experimentally assessing spatial learning and memory in reptiles is the ecological relevance of the task. Reptiles are a special taxonomic group quite distinct from rodents, demonstrating large variation in ecology, behavior, and physiology. Differences in behavior across reptilian species could possibly impact assessment of spatial cognitive abilities, particularly if the paradigm used does not tap into a natural behavior. For instance, in a species that typically seeks refuge in small crevices, spatial abilities may be easily assessed using a Barnes maze whereas this maze may not be the ideal paradigm choice in a species that typically remains motionless. Similarly, most squamate reptiles are not aquatic and thus the Morris water maze may not be a suitable choice for testing spatial learning and memory (but see15); however, this maze may be an ideal choice for testing spatial abilities in turtles16. Finally, the physiology of this group must be accounted for, as reptiles are ectothermic and proper temperature maintenance, particularly of the substrate, must be considered during the testing procedure.
The protocol and paradigm presented here were used to probe for spatial learning and memory in adult side-blotched lizards (Uta stansburiana)13, a small lizard that&#160;typically flees from predators into small crevices in rocks18. Knowing this aspect of the natural history and behavior of the species, we used a modification of the traditional Barnes maze to test for spatial learning and memory. The Barnes maze is a dry-land maze and typically used for testing spatial cognition in rodent models. We modified our maze in several ways from the rodent maze, in both design and protocol (described below). Our maze consisted of a circular platform with 10 holes equidistant from each other along the perimeter of the platform (Figure 1). The protocol described here involves a subject participating in training trials to learn the location of a goal hole, then, once the subject learns the location of the goal hole, a probe trial is used to ascertain spatial memory use during navigation to the goal.

<table><tbody><tr><td>Barnes maze</td><td>TSE Systems</td><td>302050-BM/M</td><td>Available from other vendors. Alternatively, a Barnes maze can be constructed from a standard, non-porous round table.</td></tr><tr><td>Heat tape</td><td>Big Apple Pet Supply</td><td></td><td>May also use a small space heater situated on the floor under the maze.</td></tr><tr><td>Pet keeper for small animals</td><td>Petco</td><td>1230204</td><td>Housing enclosure that can be mounted under the maze.</td></tr><tr><td>Nickel plated shelf support pegs</td><td>Newegg</td><td>241941</td><td>Pegs attached to underside of maze. Secures enclosure to maze during trials.</td></tr><tr><td>LifeCam Studio webcam</td><td>Microsoft</td><td>Q2F-00013</td><td>Available from other vendors. Other brands of webcams may also be used.</td></tr><tr><td>Tracking software</td><td>Code custom written for Matlab&lt;br/&gt; and the Image Toolbox</td><td></td><td>Video tracking software. Other tracking software such as VideoMot 2 from TSE Systems can be used.</td></tr></tbody></table>

assessing spatial learning and memory in small squamate reptiles

Clinical research has leveraged a variety of paradigms to assess cognitive decline, commonly targeting spatial learning and memory abilities. However, interest in the cognitive processes of nonmodel species, typically within an ecological context, has also become an emerging field of study. In particular, interest in the cognitive processes in reptiles is growing although experimental studies on reptilian cognition are sparse. The few reptilian studies that have experimentally tested for spatial learning and memory have used rodent paradigms modified for use in reptiles. However, ecologically important aspects of the physiology and behavior of this taxonomic group must be taken into account when testing for spatially based cognition. Here, we describe modifications of the dry land Barnes maze and associated testing protocol that can improve performance when probing for spatial learning and memory ability in small squamate reptiles. The described paradigm and procedures were successfully used with male side-blotched lizards (Uta stansburiana), demonstrating that spatial learning and memory can be assessed in this taxonomic group with an ecologically relevant apparatus and protocol.

This protocol allows for the experimental assessment of spatially based navigation in small lizards. A previous study successfully used this protocol to probe for spatial navigation in male side-blotched lizards13. In that particular study, males were trained to navigate to a goal hole and, once criterion was reached, progressed into a probe trial to assess the cues prioritized when navigating to a goal hole.
The repr...

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Assessing Spatial Learning and Memory in Small Squamate Reptiles

This paper describes a modification of the Barnes maze, a standard rodent paradigm used to assess spatial memory and learning, for use in small squamate reptiles.

Clinical research has leveraged a variety of paradigms to assess cognitive decline, commonly targeting spatial learning and memory abilities. However, ...

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7.1K Views.  Penn State Altoona. This paper describes a modification of the Barnes maze, a standard rodent paradigm used to assess spatial memory and learning, for use in small squamate reptiles.

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The Double-H Maze: A Robust Behavioral Test for Learning and Memory in Rodents

Spatial cognition research in rodents typically employs the use of maze tasks, whose attributes vary from one maze to the next. These tasks vary by their behavioral flexibility and required memory duration, the number of goals and pathways, and also the overall task complexity. A confounding feature in many of these tasks is the lack of control over the strategy employed by the rodents to reach the goal, e.g., allocentric (declarative-like) or egocentric (procedural) based strategies. The double-H maze is a novel water-escape memory task that addresses this issue, by allowing the experimenter to direct the type of strategy learned during the training period. The double-H maze is a transparent device, which consists of a central alleyway with three arms protruding on both sides, along with an escape platform submerged at the extremity of one of these arms.
Rats can be trained using an allocentric strategy by alternating the start position in the maze in an unpredictable manner (see protocol 1; &#167;4.7), thus requiring them to learn the location of the platform based on the available allothetic cues. Alternatively, an egocentric learning strategy (protocol 2; &#167;4.8) can be employed by releasing the rats from the same position during each trial, until they learn the procedural pattern required to reach the goal. This task has been proven to allow for the formation of stable memory traces.
Memory can be probed following the training period in a misleading probe trial, in which the starting position for the rats alternates. Following an egocentric learning paradigm, rats typically resort to an allocentric-based strategy, but only when their initial view on the extra-maze cues differs markedly from their original position. This task is ideally suited to explore the effects of drugs/perturbations on allocentric/egocentric memory performance, as well as the interactions between these two memory systems.

The goal of this protocol is to investigate spatial cognition in rodents. The double-H water maze is a novel test, which is particularly useful to elucidate the different components of learning, consolidation and memory, as well as the interplay of memory systems.

Spatial cognition research in rodents typically employs the use of maze tasks, whose attributes vary from one maze to the next. These tasks vary by their ...

Behavior

Using Pharmacological Manipulation and High-precision Radio Telemetry to Study the Spatial Cognition in Free-ranging Animals

An animal&#39;s ability to perceive and learn about its environment plays a key role in many behavioral processes, including navigation, migration, dispersal and foraging. However, the understanding of the role of cognition in the development of navigation strategies and the mechanisms underlying these strategies is limited by the methodological difficulties involved in monitoring, manipulating the cognition of, and tracking wild animals. This study describes a protocol for addressing the role of cognition in navigation that combines pharmacological manipulation of behavior with high-precision radio telemetry. The approach uses scopolamine, a muscarinic acetylcholine receptor antagonist, to manipulate cognitive spatial abilities. Treated animals are then monitored with high frequency and high spatial resolution via remote triangulation. This protocol was applied within a population of Eastern painted turtles (Chrysemys picta) that has inhabited seasonally ephemeral water sources for ~100 years, moving between far-off sources using precise (&#177; 3.5 m), complex (i.e., non-linear with high tortuosity that traverse multiple habitats), and predictable routes learned before 4 years of age. This study showed that the processes used by these turtles are consistent with spatial memory formation and recall. Together, these results are consistent with a role of spatial cognition in complex navigation and highlight the integration of ecological and pharmacological techniques in the study of cognition and navigation.

This paper describes a novel protocol that combines the pharmacological manipulation of memory and radio telemetry to document and quantify the role of cognition in navigation.

An animal&#39;s ability to perceive and learn about its environment plays a key role in many behavioral processes, including navigation, migration, ...

Environment

Assessing Spatial Memory Impairment in a Mouse Model of Traumatic Brain Injury Using a Radial Water Tread Maze

Despite the recent increase in use of mouse models in scientific research, researchers continue to use cognitive tasks that were originally designed and validated for rat use. The Radial Water Tread (RWT) maze test of spatial memory (designed specifically for mice and requiring no swimming) has been shown previously to successfully distinguish between controlled cortical impact-induced TBI mice and sham controls. Here, a detailed protocol for this task is presented. The RWT maze capitalizes on the natural tendency of mice to avoid open areas in favor of hugging the sides of an apparatus (thigmotaxis). The walls of the maze are lined with nine escape holes placed above the floor of the apparatus, and mice are trained to use visual cues to locate the escape hole that leads out of the maze. The maze is filled with an inch of cold water, sufficient to motivate escape but not deep enough to require that the mouse swim. The acquisition period takes only four training days, with a test of memory retention on day five and a long-term memory test on day 12. The results reported here suggest that the RWT maze is a feasible alternative to rat-validated, swimming-based cognitive tests in the assessment of spatial memory deficits in mouse models of TBI.

Here we present a protocol for a mouse-specific test of cognition that does not require swimming. This test can be used to successfully distinguish controlled cortical impact-induced traumatic brain injury mice from sham controls.

Despite the recent increase in use of mouse models in scientific research, researchers continue to use cognitive tasks that were originally designed and ...

A Behavioral Assay for Investigating the Role of Spatial Memory During Instinctive Defense in Mice

Evolution has selected a repertoire of defensive behaviors that are essential for survival across all animal species. These behaviors are often stereotyped actions elicited in response to innately aversive sensory stimuli, but their success requires enough flexibility for adapting to different spatial environments, which can change rapidly. Here, we describe a behavioral assay to evaluate the influence of learned spatial knowledge on defensive behaviors in mice. We have adapted the widely used Barnes maze spatial memory assay to investigate how mice navigate to a shelter during escape responses to innately aversive sensory stimuli in a novel environment, and how they adapt to acute changes in the environment. This new assay is an ethological paradigm that does not require training and exploits the natural exploration patterns and navigation strategies in mice. We propose that the set of protocols described here are a powerful means of studying goal-directed behaviors and stimulus-triggered navigation, which should be of interest to both the fields of instinctive behaviors and spatial memory.

This protocol describes a behavioral assay, based on the Barnes maze test, to study how instinctive defensive actions are modified by the knowledge of spatial environment.

Evolution has selected a repertoire of defensive behaviors that are essential for survival across all animal species. These behaviors are often ...

Using Enclosed Y-Mazes to Assess Chemosensory Behavior in Reptiles

Reptiles utilize a variety of environmental cues to inform and drive animal behavior such as chemical scent trails produced by food or conspecifics. Decrypting the scent-trailing behavior of vertebrates, particularly invasive species, enables the discovery of cues that induce exploratory behavior and can aid in the development of valuable basic and applied biological tools. However, pinpointing behaviors dominantly driven by chemical cues versus other competing environmental cues can be challenging. Y-mazes are common tools used in animal behavior research that allow quantification of vertebrate chemosensory behavior across a range of taxa. By reducing external stimuli, Y-mazes remove confounding factors and present focal animals with a binary choice. In our Y-maze studies, a scenting animal is restricted to one arm of the maze to leave a scent trail and is removed once scent-laying parameters have been met. Then, depending on the trial type, either the focal animal is allowed into the maze, or a competing scent trail is created. The result is a record of the focal animal&#39;s choice and behavior while discriminating between the chemical cues presented. Here, two Y-maze apparatuses tailored to different invasive reptile species: Argentine black and white tegu lizards (Salvator merianae) and Burmese pythons (Python bivittatus) are described, outlining the operation and cleaning of these Y-mazes. Further, the variety of data produced, experimental drawbacks and solutions, and suggested data analysis frameworks have been summarized.

Y-mazes enable researchers to determine the relevance of specific stimuli that drive animal behavior, especially isolated chemical cues from a variety of sources. Careful design and planning can yield robust data (e.g., discrimination, degree of exploration, numerous behaviors). This experimental apparatus can provide powerful insight into behavioral and ecological questions.

Reptiles utilize a variety of environmental cues to inform and drive animal behavior such as chemical scent trails produced by food or conspecifics. ...

Game-Based Exploration: Unlocking Human Spatial Navigation Abilities

Assessing Human Spatial Navigation in a Virtual Space and its Sensitivity to Exercise

Spatial navigation (SN) is the ability to locomote through the environment, which requires an understanding of where one is located in time and space. This capacity is known to rely on the sequential firing of place cells within the hippocampus. SN is an important behavior to investigate as this process deteriorates with age, especially in neurodegenerative disorders. However, the investigation of SN is limited by the lack of sophisticated behavioral techniques to assess this hippocampal-dependent task. Therefore, the goal of this protocol was to develop a novel, real-world approach to studying SN in humans. Specifically, an active virtual SN task was developed using a cross-platform game engine. During the encoding phase, participants navigated their way through a virtual city to locate landmarks. During the remembering phase, participants remembered where these reward locations were and delivered items to these locations. Time to find each location was captured and episodic memory was assessed by a free recall phase, including aspects of place, order, item, and association. Movement behavior (x, y, and z coordinates) was assessed through an asset available in the game engine. Importantly, results from this task demonstrate that it accurately captures both spatial learning and memory abilities as well as episodic memory. Further, findings indicate that this task is sensitive to exercise, which improves hippocampal functioning. Overall, the findings suggest a novel way to track human hippocampal functioning over the course of time, with this behavior being sensitive to physical activity training paradigms.

Author Spotlight: Investigating the Effects of Mind-Body-Movement Practices on Brain Function

Here, we present a novel, brief, and active spatial navigation task that assesses both spatial navigation and episodic memory ability. Importantly, spatial navigation and episodic memory were associated with one another, and this task demonstrated sensitivity to exercise.

Spatial navigation (SN) is the ability to locomote through the environment, which requires an understanding of where one is located in time and space. ...

Motor and Hippocampal Dependent Spatial Learning and Reference Memory Assessment in a Transgenic Rat Model of Alzheimer's Disease with Stroke

Alzheimer&#39;s disease (AD) is a debilitating neurodegenerative disease that results in neurodegeneration and memory loss. While age is a major risk factor for AD, stroke has also been implicated as a risk factor and an exacerbating factor. The co-morbidity of stroke and AD results in worsened stroke-related motor control and AD-related cognitive deficits when compared to each condition alone. To model the combined condition of stroke and AD, a novel transgenic rat model of AD, with a mutated form of amyloid precursor protein (a key protein involved in the development of AD) incorporated into its DNA, is given a small unilateral striatal stroke.
For a model with the combination of both stroke and AD, behavioral tests that assess stroke-related motor control, locomotion and AD-related cognitive function must be implemented. The cylinder task involves a cost-efficient, multipurpose apparatus that assesses spontaneous forelimb motor use. In this task, a rat is placed in a cylindrical apparatus, where the rat will spontaneously rear and contact the wall of the cylinder with its forelimbs. These contacts are considered forelimb motor use and quantified during video analysis after testing. Another cost-efficient motor task implemented is the beam-walk task, which assesses forelimb control, hindlimb control and locomotion. This task involves a rat walking across a wooden beam allowing for the assessment of limb motor control through analysis of forelimb slips, hindlimb slips and falls. Assessment of learning and memory is completed with Morris water maze for this behavioral paradigm. The protocol starts with spatial learning, whereby the rat locates a stationary hidden platform. After spatial learning, the platform is removed and both short-term and long-term spatial reference memory is assessed. All three of these tasks are sensitive to behavioral differences and completed within 28 days for this model, making this paradigm time-efficient and cost-efficient.

To investigate the co-morbid Alzheimer&#39;s disease (AD) and stroke condition in a novel model, three behavior tasks are described that assess both motor control and cognitive behaviors. These tasks include the beam-walk task, cylinder task and Morris water maze.

Alzheimer&#39;s disease (AD) is a debilitating neurodegenerative disease that results in neurodegeneration and memory loss. While age is a major risk ...

Medicine

An Open-Source Virtual Reality System for the Measurement of Spatial Learning in Head-Restrained Mice

Head-restrained behavioral experiments in mice allow neuroscientists to observe neural circuit activity with high-resolution electrophysiological and optical imaging tools while delivering precise sensory stimuli to a behaving animal. Recently, human and rodent studies using virtual reality (VR) environments have shown VR to be an important tool for uncovering the neural mechanisms underlying spatial learning in the hippocampus and cortex, due to the extremely precise control over parameters such as spatial and contextual cues. Setting up virtual environments for rodent spatial behaviors can, however, be costly and require an extensive background in engineering and computer programming. Here, we present a simple yet powerful system based upon inexpensive, modular, open-source hardware and software that enables researchers to study spatial learning in head-restrained mice using a VR environment. This system uses coupled microcontrollers to measure locomotion and deliver behavioral stimuli while head-restrained mice run on a wheel in concert with a virtual linear track environment rendered by a graphical software package running on a single-board computer. The emphasis on distributed processing allows researchers to design flexible, modular systems to elicit and measure complex spatial behaviors in mice in order to determine the connection between neural circuit activity and spatial learning in the mammalian brain.

Here, we present a simplified open-source hardware and software setup for investigating mouse spatial learning using virtual reality (VR). This system displays a virtual linear track to a head-restrained mouse running on a wheel by utilizing a network of microcontrollers and a single-board computer running an easy-to-use Python graphical software package.

Head-restrained behavioral experiments in mice allow neuroscientists to observe neural circuit activity with high-resolution electrophysiological and ...

Spatial Memory Testing Using Mazes

Spatial learning and memory are neurological functions that allow us to remember important details associated with our environment. Scientists test this phenomenon in rodents using different types of mazes like Morris water maze, Radial water maze, and Barnes maze. By investigating spatial memory in rodents, neurobehavioral scientists can gain valuable understanding of how these processes are altered following brain damage in humans.
Here, the neurobiology behind spatial memory is briefly reviewed and some general principles behind maze tests are discussed. Then, the video explains generalized protocols on how to conduct trials with specific types of mazes. Lastly, it will explain how behavioral researchers are using these tools to conduct some specific experiments.

Spatial Memory Tests Using Morris Water, Radial Water &amp; Barnes Mazes

Spatial learning and memory are neurological functions that allow us to remember important details associated with our environment. Scientists test this ...

Education

JoVE Science Education

Psychology

Behavioral Science

Barnes Maze: A Method For Assessing Spacial Learning and Memory in Lab Animals

Source: Ingersoll, J. et al. <a href="https://www.jove.com/video/58008/analyzing-spatial-learning-prosocial-behavior-mice-using-barnes-maze">Analyzing Spatial Learning and Prosocial Behavior in Mice Using the Barnes Maze and Damsel-in-Distress Paradigms</a>. J. Vis. Exp. (2018).
This video shows how to use the Barnes Maze with a mouse. The Barnes Maze testing procedure is used by researchers to assess spatial learning and memory in laboratory animals following repeated trials.

Source: Ingersoll, J. et al. Analyzing Spatial Learning and Prosocial Behavior in Mice Using the Barnes Maze and Damsel-in-Distress Paradigms. J. Vis. ...