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Induction of an Inflammatory Response in Primary Hepatocyte Cultures from Mice

DOI :

10.3791/55319-v

March 10th, 2017

March 10th, 2017

9,702 Views

1Katz Family Drug Discovery Center and Division of Nephrology and Hypertension, Department of Medicine, University of Miami Leonard M. Miller School of Medicine, 2Department of Cell Biology and Anatomy, University of Miami Leonard M. Miller School of Medicine

Here, we show an enzymatic approach to isolate primary hepatocytes from adult mice, and we describe the quantification of an inflammatory response using ELISA and real-time PCR.

Tags

Inflammatory Response

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