Isolation of Primary Mouse Hepatocytes for Nascent Protein Synthesis Analysis by Non-radioactive L-azidohomoalanine Labeling Method
18.8K Views
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08:04 min
•
October 23rd, 2018
DOI :
October 23rd, 2018
•Esam S.B. Salem*1,2, Kazutoshi Murakami*2, Toshimasa Takahashi2, Elise Bernhard2, Vishnupriya Borra2, Mridula Bethi2, Takahisa Nakamura2,3,4
1Department of Pharmacology and Systems Physiology, College of Medicine, University of Cincinnati, 2Division of Endocrinology, Cincinnati Children's Hospital Medical Center, 3Division of Developmental Biology, Cincinnati Children's Hospital Medical Center, 4Department of Pediatrics, College of Medicine, University of Cincinnati
Chapters in this video
0:04
Title
0:47
Liver Perfusion and Extraction
2:45
Primary Mouse Hepatocyte Isolation
4:07
Polyacrylamide Gel Electrophoresis (PAGE) Analysis
6:06
Results: Representative Nascent Protein Expression Analyses
7:31
Conclusion
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