An Advanced Murine Model for Nonalcoholic Steatohepatitis in Association with Type 2 Diabetes

DOI :

10.3791/59470-v

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April 26th, 2019

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6,793 Views

Julia Sbierski-Kind^1,2,3,4, Katharina Schmidt-Bleek^3,6, Mathias Streitz^3,5, Jonas Kath^3,5, Joachim Spranger^1,2,4*, Hans-Dieter Volk^2,3,5*

¹Department of Endocrinology & Metabolism Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, ²Berlin Institute of Health (BIH), ³Berlin-Brandenburg Center for Regenerative Therapies (BCRT), Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, ⁴DZHK (German Centre for Cardiovascular Research), ⁵Institute of Medical Immunology, Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, ⁶Julius Wolff Institute (JWI) and Center for Musculoskeletal Surgery, Charité - Universitätsmedizin Berlin, Berlin, Germany, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin

Chapters

A simple and reliable diet-induced rodent animal model for nonalcoholic steatohepatitis (NASH) is described, achieved through non-SPF housing of the animals and administration of a specific high-fat diet. We describe identification of hepatic and adipose immune cell subsets to recapitulate human immunological conditions by exposing mice to environmental germs.

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