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Isolating and Analyzing Cells of the Pancreas Mesenchyme by Flow Cytometry

DOI :

10.3791/55344-v

5:38 min

January 28th, 2017

January 28th, 2017

11,652 Views

1Cell and Developmental Biology, Sackler Faculty of Medicine, Tel Aviv University

Here, we describe a method for the isolation of cells in the pancreas microenvironment from embryonic, neonatal and adult mouse tissue, focusing on the isolation of mesenchymal cells. This method allows profiling of cell gene expression and protein secretion in order to elucidate the extrinsic signals that regulate pancreas development, function, and tumorigenesis.

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Keywords Pancreas

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