Development of Recombinant Proteins to Treat Chronic Pain

9.4K Views

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10:37 min

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April 11th, 2018

DOI :

10.3791/57071-v

April 11th, 2018

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Judith Prado¹, Jelena Popov-Celeketic², Cristine Steen-Louws¹, Ramin Raoof¹, Eric Hack¹, Niels Eijkelkamp¹^,³

¹Laboratory of Translational Immunology, University Medical Center Utrecht, Utrecht University, ²Department of Rheumatology & Clinical Immunology, University Medical Center Utrecht, Utrecht University, ³Laboratory of Neuroimmunology and Developmental Origins of Disease, University Medical Center Utrecht, Utrecht University

Summary

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Recombinant Proteins

IL4 10 Fusion Protein

Chronic Pain

Anti inflammatory Cytokines

Affinity Chromatography

Dialysis

SDS PAGE

Chapters in this video

0:05

Title

1:15

Cell Transfection, Protein Purification, and Affinity Chromatrography

3:49

Bioactivity of IL4-10 Fusion Protein

4:46

Induction of Chronic Inflammatory Pain in Mice. Pain Measurements: The Von Frey Test: Mechanical Sensitivity

5:52

Pain measurements: Hargreaves Test for Thermal Sensitivity Dynamic Weight Bearing Test for Postural Deficits

6:48

Intrathecal Injection of IL4-10 Fusion Protein for Analgesia

7:56

Results: Persistent Inflammatory Pain Reduction Using IL4-10 Fusion Protein

9:38

Conclusion

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