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Peptide:MHC Tetramer-based Enrichment of Epitope-specific T cells

DOI :

10.3791/4420-v

October 22nd, 2012

October 22nd, 2012

17,497 Views

1Center for Immunology and Inflammatory Diseases, and Pulmonary and Critical Care Unit, Massachusetts General Hospital and Harvard Medical School

This protocol describes the use of peptide:MHC tetramers and magnetic microbeads to isolate low frequency populations of epitope-specific T cells and analyze them by flow cytometry. This method enables the direct study of endogenous T cell populations of interest from in vivo experimental systems.

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