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Quantifying Agonist Activity at G Protein-coupled Receptors

DOI :

10.3791/3179-v

December 26th, 2011

December 26th, 2011

18,048 Views

1Department of Pharmacology, University of California, Irvine, 2Department of Pharmacology, University of California, 3Schmid College of Science, Chapman University

A method for estimating the affinity constant of an agonist for the active state (Kb) of a G protein-coupled receptor is described. The analysis provides absolute or relative measures of Kb depending on whether constitutive receptor activation is measurable. Our method applies to various responses downstream from receptor activation.

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