JoVE Logo
Faculty Resource Center

Sign In

Freeze-Fracture Electron Microscopy for Extracellular Vesicle Analysis

DOI :

10.3791/63550-v

11:30 min

September 16th, 2022

September 16th, 2022

2,712 Views

1Faculty of Medicine, Institute of Cell Biology, University of Ljubljana

We present a protocol for the isolation and freeze-fracturing of extracellular vesicles (EVs) originating from cancerous urothelial cells. The freeze-fracture technique revealed the EVs' diameter and shape and-as a unique feature-the internal organization of the EV membranes. These are of immense importance in understanding how EVs interact with the recipient membranes.

Tags

Freeze fracture Electron Microscopy

-- Views

Related Videos

article

Transmitochondrial Cybrid Generation Using Cancer Cell Lines

article

Optimization of Performance Parameters of the TAGGG Telomere Length Assay

article

Shear Assay Protocol for the Determination of Single-Cell Material Properties

article

Multiplex Immunofluorescence Combined with Spatial Image Analysis for the Clinical and Biological Assessment of the Tumor Microenvironment

article

Author Spotlight: Exploring the Role of Ion Channels in Cancer: Characterization and Potential Treatment Approaches

article

A Three-Dimensional Technique for the Visualization of Mitochondrial Ultrastructural Changes in Pancreatic Cancer Cells

article

Modeling Brain Tumors In Vivo Using Electroporation-Based Delivery of Plasmid DNA Representing Patient Mutation Signatures

article

Magnetic Fluorescent Bead-Based Dual-Reporter Flow Analysis of PDL1-Vaxx Peptide Vaccine-Induced Antibody Blockade of the PD-1/PD-L1 Interaction

article

Author Spotlight: Demonstrating Systematic Endobronchial Ultrasound to New Endoscopists

article

Author Spotlight: Creating Human Vascularized Micro-Tumors as Models for Translational Cancer Research

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved