February 6th, 2021
•In this protocol, we describe how to generate murine primary epithelial colon monolayers directly from intestinal crypts. We provide experimental approaches to generate confluent monolayers on permeable filters, confluent monolayers for scratch wound healing and biochemical studies, and sparse and confluent monolayers for immunofluorescence analysis.
Related Videos
The Preparation of Primary Hematopoietic Cell Cultures From Murine Bone Marrow for Electroporation
Primary Human Bronchial Epithelial Cells Grown from Explants
Isolation of Primary Myofibroblasts from Mouse and Human Colon Tissue
Cryosectioning Method for Microdissection of Murine Colonic Mucosa
Intestinal Epithelial Regeneration in Response to Ionizing Irradiation
Primary Culture of Porcine Retinal Pigment Epithelial Cells
Culture of Piglet Intestinal 3D Organoids from Cryopreserved Epithelial Crypts and Establishment of Cell Monolayers
The 3D Culturing of Organoids from Murine Intestinal Crypts and a Single Stem Cell for Organoid Research (Video) | JoVE
Studying the Epithelial Effects of Intestinal Inflammation In Vitro on Established Murine Colonoids (Video) | JoVE
Generation and Manipulation of Rat Intestinal Organoids (Video) | JoVE
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved