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Generation of Murine Primary Colon Epithelial Monolayers from Intestinal Crypts

DOI :

10.3791/62156-v

6:57 min

February 6th, 2021

February 6th, 2021

5,836 Views

1Department of Pathology, School of Medicine, University of Michigan

In this protocol, we describe how to generate murine primary epithelial colon monolayers directly from intestinal crypts. We provide experimental approaches to generate confluent monolayers on permeable filters, confluent monolayers for scratch wound healing and biochemical studies, and sparse and confluent monolayers for immunofluorescence analysis.

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Murine Primary Colon Epithelial Monolayers

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