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Medium-scale Preparation of Drosophila Embryo Extracts for Proteomic Experiments

DOI :

10.3791/55804-v

May 30th, 2017

May 30th, 2017

7,667 Views

1Biology Department, University of Massachusetts Boston, 2Department of Bioengineering, University of California, San Diego

The goal of this protocol is to provide a straightforward and inexpensive approach to collecting Drosophila embryos at medium scale (0.5-1 g) and preparing protein extracts that can be used in downstream proteomic applications, such as affinity purification-mass spectrometry (AP-MS).

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Drosophila Embryo Extracts

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