Preparation of 5 Liter and 1 Liter Fly Cages and Collection of Fly Embryos
4:12
Embryo Dechorionation
5:15
Preparation of Embryo Extract
7:15
Results: Preparation of Drosophila Embryo Extracts for Proteomics Experiments
8:35
Conclusion
Transkript
The overall goal of this experiment, is to provide a straightforward and inexpensive approach to collecting Drosophilia Embryos at medium scale. And preparing protein extracts that can be used in downstream Proteomic applications, such as affinity
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The goal of this protocol is to provide a straightforward and inexpensive approach to collecting Drosophila embryos at medium scale (0.5-1 g) and preparing protein extracts that can be used in downstream proteomic applications, such as affinity purification-mass spectrometry (AP-MS).